preparation of 50 ml chloroform water B.P.

Object: To prepare and submit 50 ml of chloroform water BP.

Reference-

1. British pharmacopoeia 2004 vol-III p.n. 2321.

1. Carter S.J., “copper and gunn’s, Tutorial pharmacy. CBS publishers and distributors. New Delhi-03 India, page no-49, 69, 364.

Requirement-

Chemical-chloroform,

Apparatus- pipette, beaker 100 ml, funnel, vol. flask, measuring cylinder.

Theory: Chloroform water is a category of aromatic water. Chloroform is a clear, odorless, mobile liquid and has burning sweet taste. Specific gravity of chloroform is between 1.474 to 1.478. Proper handling of chloroform is must because in presence of flame or light it produced harmful gases like hydrogen chloride and phosgene gas. The small amount of ethanol in chloroform is retards the formation of carbonyl chloride (COCl2). It is rarely used as an inhalation anesthetic due to its serious side effect on heart and liver. Internally it is used as carminative and externally as an irritant. In the preparation of chloroform water vigorous shaking is necessary to sub divided the chloroform in small globules to exchange the solubility.

Procedure:

·         All the apparatus were thoroughly washed and dried.

·         All the ingredients were weighed accurately.

·         Required quantity of chloroform was mixed with purified water in a conical flask by vigorous shaking.

·         This semi-saturated solution transferred in a clean amber color tightly closed container with proper labeling.

Category: Pharmaceutical aid

Dose:           15 to 20 ml

Storage: store in a well closed container and protected from light.

Use: Preservative, as a vehicle and general anesthetic

Observation table:

S. No.	Ingredients	Quantity given	Quantity taken
1	Chloroform	2.5 ml	0.125ml
2	Purified water	q.s. up to 1000 ml	q.s. up to 50ml

Label:

CHLOROFORM WATER BP 50 ML

Composition-                                                                                                                                                          Batch No. Dose                                                                       MFG Date Category                                                                 Exp Date Storage                                                                   MRP-                                                                      (inclusive of all taxes)

MFD By-

Result: 50 ml Chloroform water BP was prepared and submitted.